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A synchronous papillary and follicular thyroid carcinoma presenting as a large toxic nodule in a female adolescent
International Journal of Pediatric Endocrinology volume 2020, Article number: 14 (2020)
We report for the first time a synchronous papillary and follicular thyroid carcinoma in a 12-year-old girl presenting with a large (5 cm diameter) left thyroid nodule, an increased left and right upper pole technetium tracer uptake at scintigraphy and hyperthyroidism. The uptake at the right lobe was explained by the crossing of the left nodule to the right site of the neck at Computed Tomography (CT) scanning.
Although thyroid nodules are less common in children than in adults, there is more vigilance required in children because of the higher risk of malignancy. According to literature, about 5% of the thyroid nodules in adults are malignant versus 20–26% in children. The characteristics of 9 other pediatric cases with a differentiated thyroid carcinoma presenting with a toxic nodule, which have been reported during the last 20 years, are summarized. A nodular size of more than 3.5 cm and female predominance was a common finding.
The presence of hyperthyroidism in association with a hyperfunctioning thyroid nodule does not rule out thyroid cancer and warrants careful evaluation, even in the absence of cervical lymph node invasion.
Solitary thyroid nodules are rare in childhood in comparison with adulthood, but have a higher risk of malignancy . Up to 26% of the thyroid nodules in children were found to be malignant [1,2,3]. Most differentiated thyroid cancers (DTC) in children are papillary carcinoma, which only very rarely (over) produce thyroid hormones, causing subclinical or overt hyperthyroidism . In a recent pediatric cohort study of thyroid nodules, hyperthyroidism was found in only 5% . The finding of a hyperfunctioning or hot nodule on scintigraphy in the context of hyperthyroidism, especially in the absence of enlarged cervical lymph nodes, is usually reassuring, as in most cases a benign follicular adenoma is diagnosed [5,6,7].
We additionally reviewed the previously reported DTC in children and adolescents with a hyperfunctioning thyroid nodule associated with hyperthyroidism, also called toxic nodule, to look for common characteristics and potential risk factors for malignancy. A literature search on Pubmed and Web of Science was performed in the English literature in a period from January 2009, when most laboratories were using a third generation TSH assay, until December 2019. The search terms used were “hyperthyroidism and thyroid carcinoma”, “toxic nodule”, “hyperfunctioning nodule and thyroid carcinoma” in conjunction with either “children” or “pediatric”. Inclusion criteria were detailed case descriptions of children aged below 16 years old with a clinical and biological hyperthyroidism and an underlying differentiated thyroid carcinoma documented at histological examination. Finally, 8 articles describing 9 cases were selected. Clinical characteristics, biological, imaging and histological results and initial treatment of these 9 pediatric patients are summarized in Table 1.
Overview of previously reported case reports of DTC in children with a toxic nodule [8,9,10,11,12,13,14,15]
All described patients were female except one. Age at presentation varied between a minimum age of 2 months and a maximum age of 16 years (median 11 years). The severity of the hyperthyroidism is not always mentioned, but FT4 concentrations up to 3 times the upper limit with less elevated FT3 concentrations were reported. Right as well as left sided localisation of the nodule was seen, while the longest diameter of the nodule ranged from 35 to 50 mm. Suppressed activity in surrounding tissue at scintigraphic evaluation was noted in 8 cases. In four cases a follicular variant of a papillary thyroid carcinoma was found, while in the others either a papillary thyroid carcinoma or a follicular carcinoma was present (Table 1).
In a 12-year-old female, presenting with a painless neck swelling since one month, a 5 cm long, non-tender but firm nodule in the left thyroid lobe was detected at physical examination. There was no palpable cervical lymphadenopathy. Slight tachycardia (pulse rate 95/min), but no exophthalmos was present. The patient reported intermittent sore throat, increasing nervousness and a 2 kg weight loss in the last several months. There was no history of radiation exposure. Her mother had recently undergone surgery for a multi-nodular goitre. There was no family history of cancer or intestinal polyps.
Thyroid function tests showed an elevated FT4 (51 pmol/L or 39.6 pg/mL) and thyroglobulin concentration (435 μg/L) and a decreased TSH (< 0.005 mU/L) concentration.Footnote 1 Serum anti-thyroglobulin, anti-thyroidperoxidase and anti-TSH receptor antibodies were undetectable. Doppler ultrasound showed a normal right lobe as well as a sharp defined hypervascular solid multilobular mass (longest diameter 53 mm) with cystic components with microcalcifications in the left lobe. A 99mTechnetium scintigraphy (Fig. 1) showed a global, but heterogeneous hyperfunctioning thyroid gland with excessive uptake at the upper left lobe and upper right lobe. A right tracheal deviation by the left thyroid mass, but no cervical or mediastinal lymphnodes or lung masses, was seen on the CT scan of neck and thorax (Fig. 2). Subsequently, a large toxic adenoma crossing the midline was diagnosed, explaining the tracer uptake in the right upper pole region. Fine needle biopsy was refused by the patient and a left thyroidectomy was proposed. The patient underwent a left hemithyroidectomy after 2 months of methimazole therapy, which resulted in normalized thyroid function in one month’s time. Histological examination of the left lobe showed two separated morphologically distinct neoplastic lesions arising in what appeared to be a multinodular goitre. The largest lesion (measuring 2.9 cm) showed a follicular growth pattern with cytonuclear atypia, multiple foci of intracapsular vascular invasion and some foci of capsular invasion. Separated from this lesion by a couple of millimetres, a second neoplastic lesion was seen. This was non-encapsulated and infiltrative and had a papillary and follicular growth pattern with cytonuclear features of a papillary thyroid carcinoma (nuclear clearing, nuclear inclusions and grooves). The non-encapsulated, infiltrative neoplastic lesion with papillary architecture and cytomorphology showed aberrant apical and strong HBME immunohistochemical staining, whereas the adjacent lesion with follicular growth pattern was completely negative for HBME. Therefore, based on the morphology and immunohistochemistry, a diagnosis of a synchronous minimally invasive follicular thyroid carcinoma (with multifocal capsular invasion and angioinvasion) and a non-encapsulated, infiltrative (classical type) papillary thyroid carcinoma of the left lobe was made (Fig. 3). Consequently, total thyroidectomy was performed.
Genetic testing on the surgical specimen was negative for BRAF and NRAS mutations and RET/PTC rearrangements. Results of TSHR gene, GNAS gene and PTEN gene analysis were normal.
Hyperfunctioning nodules at thyroid scintigraphy, also called hot nodules, can present with or without hyperthyroidism. In the latter case, these nodules are also described as toxic nodules in literature. In the previously reported nine pediatric cases of DTC with associated hyperthyroidism and a hot nodule at scintigraphy, follicular carcinomas, papillary carcinomas as well as follicular variants of papillary thyroid carcinoma were diagnosed. We report for the first time a synchronous papillary-follicular thyroid carcinoma in female adolescent presenting with a toxic nodule. In our case as well as in the previously reported pediatric cases, the nodules were found to be greater than 3 cm in diameter, suggesting that clinical hyperthyroidism does not appear until the nodule is at least 3 cm in diameter. When comparing with non-hyperfunctioning nodules, thyroid nodules in hyperthyroid adolescents were found to have more compressive signs and a greater nodule size, and are mostly diagnosed as follicular adenomas (toxic adenoma) [5, 16].
The major goal of the diagnostic evaluation of thyroid nodules is to differentiate thyroid cancers, especially aggressive lesions, from benign adenomas. In the initial work-up of a thyroid nodular lesion, thyroid function tests are usually performed. The American Thyroid Association (ATA) Taskforce recommends that patients who have a thyroid nodule larger than 1 to 1.5 cm in any dimension, should have a serum thyrotropin (TSH) measurement . If hyperthyroidism is associated with a nodule on ultrasound, a scintiscan is the next logical step to document the hyperfunctioning of the nodule, especially when thyroid stimulating immunoglobulines are absent. In toxic adenoma, the typical scintigraphic finding is a hot pattern in the nodule with the remnant thyroid tissue showing a severely decreased or absent uptake . In our case no complete suppression was found, while in the other pediatric cases both complete and incomplete scintigraphic suppression patterns were reported. An incomplete suppression pattern was seen as a risk factor for DTC by Niedziela et al.  in his series of 31 children with a hyperfunctioning nodule.
The prevalence of malignancy in a hot nodule in adults has been estimated at 3.1% . Histological outcome studies in children with a toxic nodule are very limited. No malignancy was detected in 6 Italian hyperthyroid pediatric patients with a solitary toxic nodule at surgery . In an American study of 4 children with a hot or warm nodule and persisting T3 hyperthyroidism, no malignancy was found after partial thyroidectomy , while in another study of 2 hyperthyroid adolescents a follicular carcinoma was found in one female . However, in a more recent study of 15 Polish children with hyperthyroidism and a hyperfunctioning nodule at scintigraphy, a DCT was diagnosed in 2 children after surgery . In none of the reported adult or pediatric cases a simultaneous papillary and follicular carcinoma in a hot nodule was described. The simultaneous occurrence of different types of thyroid cancer in a single patient is very rare. Although there are noticeable reports about synchronous papillary cancer, the reports of simultaneous papillary and follicular cancer are actually rare . This simultaneous thyroid tumor presentation has been described as coincidental in the literature as no common gene mutation for the pathogenesis of the different tumor types of the thyroid has been found. Mixed tumors however can occur as part of familial cancer syndromes. Cowden’s syndrome was excluded in our patient by genetic analysis, while no signs of Carney’s complex were present. Fine needle aspiration (FNA) biopsy is considered to be the most accurate procedure to identify malignant nodules, but is generally not advised in hot nodules. First, their larger size might easily result in false negative results. Second, hot nodules have lower likelihood of finding malignancy compared to cold nodules and third, there are histological difficulties in differentiating follicular adenoma from follicular thyroid carcinoma.
Contrary to guidelines for adults, the current recommendation for treatment of hyperfunctioning nodules in children is surgical resection rather than radio iodine treatment [11, 24]. Surgery is even more preferred when there are signs of compression, which is a common finding in hyperfunctioning nodules since they are larger in most cases than non-functional nodules . Because of the absence of clinical (male gender, age < 10 years, family history of thyroid cancer) and ultrasound (irregular margins) risk factors, as well as the absence of enlarged cervical lymph nodes, mediastinal and lung invasion at CT scanning, DCT was initially not suspected in this case and a hemithyroidectomy was performed with a the preoperative diagnosis of a large toxic adenoma. In the presence of a size of more than 4 cm and an intense internal vascularisation (and calcifications in the nodule), an initial total thyroidectomy could have been justified, as suggested by Deluca et al. . On the other hand, a family history of multinodular goitre and the absence of cervical and lung invasion was assessed as a reassuring feature in our case, favouring a left hemithyroidectomy. We first treated our patient with methimazole, as patients with overt hyperthyroidism due to a hyperfunctioning nodule should be euthyroid prior to the surgical procedure . In all reported cases, euthyroidism was observed relatively quickly after medical treatment, as observed in our case.
Somatic mutations of the TSHR and GNAS1 gene have been detected in adolescents presenting with autonomous functioning benign thyroid nodule as well as thyroid carcinoma with or without associated hyperthyroidism [8, 14, 22]. In one study, in 17 of 29 of benign hyperfunctioning nodules somatic TSHR mutations were found, while only one of the 4 studied DCT a mutation was found. Surgical specimen examination in our case was negative for BRAF and NRAS mutations and RET/PTC rearrangements. Increased malignancy rate of hyperfunctioning nodules was found not to be associated with BRAF, NRAS mutations and PAX8/PPARG and RET/PTC rearrangements .
In conclusion, our case illustrates the difficulty to accurately determine the risk of DTC in hyperthyroid adolescents presenting with a toxic nodule and provides histological evidence that a large thyroid carcinoma can be composed of both a follicular and papillary carcinoma.
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Data sharing is not applicable to this article as no datasets were generated or analysed during the current study.
Reference rate: FT4 12.0–22.0 pmol/L, thyroglobulin concentration 0.2–70.0 μg/L, TSH 0.70–6.4 mU/L
American Thyroid Association
Differentiated Thyroid Cancer
Thyroid Stimulating Hormone
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Van Vlaenderen, J., Logghe, K., Schiettecatte, E. et al. A synchronous papillary and follicular thyroid carcinoma presenting as a large toxic nodule in a female adolescent. Int J Pediatr Endocrinol 2020, 14 (2020). https://doi.org/10.1186/s13633-020-00084-4
- Toxic nodule
- Follicular thyroid carcinoma
- Papillary thyroid carcinoma