Volume 2015 Supplement 1

Abstracts from the 8th APPES Biennial Scientific Meeting

Open Access

A single antenatal course of betamethasone adversely affects glucose regulation in adulthood and the next generation in childhood

  • Sarah Mathai1,
  • José Derraik1,
  • Wayne Cutfield1,
  • Stuart Dalziel1,
  • Janene Biggs1,
  • Craig Jefferies1,
  • Jane Harding1 and
  • Paul Hofman1
International Journal of Pediatric Endocrinology20152015(Suppl 1):O22

https://doi.org/10.1186/1687-9856-2015-S1-O22

Published: 28 April 2015

Objective

To assess whether a single antenatal course of betamethasone affects insulin sensitivity and other metabolic parameters in the offspring, and whether effects are transmitted to the next generation.

Methods

A cohort of 52 adults (aged 35.7 years, 46% men, 23 born after steroid treatment) and their term-born children (n=61, aged 8.0 years, 52% boys, 49% from a parent born after steroid treatment), was recruited in Auckland. Insulin sensitivity and secretion were assessed using hyperglycaemic clamps in adults, and HOMA-IR in children. Other assessments included DXA-derived body composition, lipid profile, adipokines, and 24-hour ambulatory blood pressure monitoring.

Results

Insulin sensitivity over the last 60 minutes of the hyperglycaemic clamp was 31% lower in the Steroid group (p=0.048), with a similar trend for overall insulin sensitivity (p=0.061). Steroid adults had a compensatory increase in first-phase insulin that was 53% higher than in controls (p=0.031), with total insulin secretion 44% higher in the Steroid group (p=0.044). Children of parents born after steroid treatment had higher fasting glucose (p=0.049) and insulin (p=0.008) concentrations than controls. HOMA-IR values indicated that children in the Steroid group were more insulin resistant than controls (p=0.006).
Table 1

Study outcomes in the offspring (adults, F1) born from mothers who were either treated with antenatal betamethasone (Steroid) or not treated (Control), and in subsequent generation (children, F2). Data are means and 95% CI, adjusted for confounders.

  

Steroid

Control

p-value

Adults (F1)

n

23

29

 
 

1st phase insulin (mU/l)

43.1 (30.9–60.2)

28.1 (20.9–37.8)

0.031

 

2nd phase insulin (mU/l)

57.5 (42.6–77.8)

41.6 (31.8–54.4)

0.068

 

Total insulin (mU/l)

101.7 (74.7–138.4)

70.4 (53.5–92.6)

0.044

 

Insulin sensitivity

16.1 (11.0–22.4)

23.1 (17.3–30.2)

0.061

 

Insulin sensitivity last 60 min

14.3 (9.9–19.7)

20.6 (15.7–26.6)

0.048

Children (F2)

n

30

31

 
 

Fasting glucose (mg/dl)

4.88 (4.69–5.12)

4.67 (4.50–4.85)

0.049

 

Fasting insulin (mU/l)

5.05 (3.98–6.41)

3.57 (2.79–4.58)

0.008

 

HOMA-IR

1.10 (0.85–1.43)

0.74 (0.57–0.98)

0.006

Conclusion

This study shows that maternal treatment with a single dose of betamethasone is associated with reduced insulin sensitivity in the offspring in mid-adulthood. Importantly, there is indication of an inter-generational effect, with the subsequent generation displaying increased insulin resistance.

Authors’ Affiliations

(1)
Liggins Institute, University of Auckland

Copyright

© Mathai et al; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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