Volume 2015 Supplement 1

Abstracts from the 8th APPES Biennial Scientific Meeting

Open Access

Abundant CD4+FOXP3+ regulatory T cells fail to suppress the proliferation of T cells in patients with Turner syndrome

  • Young Ah Lee1,
  • Hang-Rae Kim2, 3,
  • Jeong Seon Lee1,
  • Haewoon Jung1,
  • Hwa Young Kim1,
  • Kyung Min Lee1,
  • Ji Hyun Sim2,
  • Doo Hyun Chung3, 4, 5,
  • Choong Ho Shin1 and
  • Sei Won Yang1
International Journal of Pediatric Endocrinology20152015(Suppl 1):O25

https://doi.org/10.1186/1687-9856-2015-S1-O25

Published: 28 April 2015

Context

Why Turner syndrome (TS) patients are predisposed to autoimmune disease remains unclear.

Objective

We investigated whether the frequency, phenotype, and suppressive function of CD4+FOXP3+ regulatory T cells (Tregs) are altered in young TS patients with the 45,X karyotype compared to age-matched controls.

Methods

Peripheral blood mononuclear cells from young TS patients (n = 24, 17.4–35.9 yrs) and controls (n = 29) were stained with various Treg markers to characterize their phenotypes. Tregs sorted for CD4+CD25bright were co-cultured with autologous CD4+CD25 target cells in the presence of anti-CD3 and CD28 antibodies to assess their suppressive function.

Results

TS patients exhibited a higher frequency of CD4+FOXP3+ Tregs among their lymphocytes (mean 2.06 vs. 1.52%, P = 0.005) and FOXP3+ Tregs among their CD4+ T cells (7.44 vs. 4.19%, P < 0.001) compared to controls. The expression of inhibitory CTLA-4 in the Tregs of TS patients was also significantly higher (mean fluorescence intensity = 214.1 vs. 184.6, P = 0.003). The frequency of Tregs expressing GITR+, CXCR3+, and CCR4+CCR6+ was comparable between the two groups. However, the ability of Tregs to suppress the in vitro proliferation of autologous CD4+CD25 T cells was significantly impaired in TS patients compared to controls (P < 0.05 at a 0.1:1 ratio of Tregs to target cells, P < 0.01 at 0.25:1, 0.5:1, and 1:1).

Conclusions

The Tregs of TS patients could not efficiently suppress the proliferation of autologous effector T cells, despite the abundance of Tregs in the peripheral circulation.

Authors’ Affiliations

(1)
Department of Pediatrics, Seoul National University Children's Hospital
(2)
Department of Anatomy, Seoul National University College of Medicine
(3)
Department of Biomedical Sciences, Seoul National University College of Medicine
(4)
Department of Pathology, Seoul National University Hospital
(5)
Ischemic/Hypoxia Institute, Seoul National University College of Medicine

Copyright

© Ah Lee et al; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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