Genetic susceptibility of serum cholesterol and triglyceride in Chinese Han children and adolescents

Objective

Genetic studies might provide new insights into the biological mechanisms underlying lipid metabolism and risk of Cardiovascular disease. We therefore conducted a study to identify genetic determinants of triglycerides(TG), high-density lipoprotein cholesterol (HDL-C) and non-high-density lipoprotein cholesterol (non-HDL-C).

Methods

We investigated adiponectin receptor 1(AdipoR1) gene rs10920533 associated with non-HDL-C(case: 109 subjects with high non-HDL-C; control: 701 subjects with normal non-HDL-C); apolipoprotein A5(ApoA5) gene -1131T>C and -3A>G associated with TG(case: 245 subjects with high TG; control: 595 subjects with normal TG); glucocorticoid receptor(GR) gene rs12521436 associated with HDL-C(case: 129 subjects with low HDL-C; control: 722 subjects with normal HDL-C). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used for SNP genotyping.

Results

Compared to AdipoR1 rs10920533 GG genotype, carriers of AG had lower non-HDL-C, but the differences did not reach statistical significance; after adjusted for BMI, TC, TG and LDL-C with multi-factor logistic regression, AdipoR1 rs10920533 AG genotype was not independently associated with the non-HDL-C level. APOA5 -1131CC and -3GG had higher TG compared to common allele homozygotes, respectively, but the differences did not reach statistical significance; after adjusted for age, WC, TC, HDL-C and LDL-C with multi-factor logistic regression, the risk of -1131CC genotype was found significantly increased than that of the TT genotype, the OR value of which was 2.667(95% confidence interval: 1.413-5.033) and the risk of -3GG genotype was found significantly increased than that of the AA genotype, the OR value of which was 2.561(95% confidence interval: 1.342-4.888). For GR rs12521436, compared to the homozygous for the G allele, carriers of A allele had lower levels of HDL-C, but the differences did not reach statistical significance; after adjusted for BMI, TC and TG with multi-factor logistic regression the result show that a more increased risk for lower HDL-C in subjects with the A carriers, which the crude OR for subjects with A allele was 1.937 (95% confidence interval: 1.166-3.215) relative to GG carriers.

Conclusion

Our results demonstrate an independent risk for ApoA5 -1131T>C and -3A>G gene polymorphisms in the development of a elevated TG level. GR rs12521436 gene polymorphisms might contribute to a reduced level of HDL-C. The single nucleotide polymorphism rs10920533 in the AdipoR1 gene is not associated with non-HDL-C level.