Fig. 5

Mutational analysis of the ABCC8 gene. a DNA obtained from the peripheral blood leukocytes of the patient. A c.2506C > T mutation was detected in the heterozygous state with the wild type allele. The mutation was found to be of paternal origin (data not shown). b DNA obtained from the focus by laser microdissection. A loss of the wild type maternal allele was evident. The reduced peak of the wild type allele probably reflects the normal tissue within the focus