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Table 3 Protein spot displaying intensity differences (p < 0.05) between insulin resistant (IR) and non-IR obese children before FDR analysis

From: Proteomic analysis allows for early detection of potential markers of metabolic impairment in very young obese children

Spot

Spot intensity in IR,

MS

MS

MS

MS -MS

MS-MS

MS-MS

Identification

Significance level

Score/Cut-off

Matched fragments

Sequence coverage (%)

Score/Cut-off

Matched fragments

Sequence coverage (%)

1703

Upregulated, p < 0.05

111/66

15

54

100/40

6(4)

26

Alpha-1-Antitrypsin

3002

Upregulated, p < 0.05

48/66

4

--

52/38

1(1)

4

Haptoglobin

3101

Downregulated, p < 0.05

160/66

20

69

116/40

11(8)

43

Apolipoprotein A1

3301

Downregulated, p < 0.05

85/66

7

22

79/41

5(4)

23

Clusterin (Apo J)

4101

Downregulated, p < 0.01

165/66

23

73

150/49

11(5)

43

Apolipoprotein A1

4103

Downregulated, p < 0.05

161/66

22

73

171/59

11(4)

43

Apolipoprotein A1

4402

Downregulated, p < 0.05

49/66

5

--

97/58

1(1)

4

Clusterin (Apo J)

4503

Downregulated, p < 0.05

93/66

13

33

72/59

6(2)

15

Haptoglobin

5503

Downregulated, p < 0.05

56/66

6

--

81/59

2(0)

4

Haptoglobin

7101

Upregulated, p < 0.05

74/66

6

20

99/56

2(1)

7

Haptoglobin

8101

Upregulated, p < 0.05

55/66

5

--

73/56

2(1)

13

Haptoglobin

9101

Upregulated, p < 0.05

49/66

3

--

105/55

1(1)

4

Haptoglobin

  1. The second column shows the direction of the change observed in IR vs. non-IR. Abbreviations: MS Mass spectrometry, MS-MS Tandem mass spectrometry.
  2. Spots in bold remained significant after FDR analysis.